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She has concurrently taught at Makerere University College of Health Sciences, which uses Mulago Hospital as its teaching hospital. She has risen through the ranks, over the years, from research associate, assistant lecturer, lecturer, senior lecturer, to her current rank of Associate Professor, as of August 2018. She has also worked at the University's Infectious Diseases Institute.

In 2017, Pauline Byakika, MBChB, MMed (Medicine), MSc, PhD, was elected to serve as the Vice President of the Uganda Medical Association, for the next two years. In addition to her duties, as a member of the nine-person Executive Committee, she is a member of the 55-person National Governing Council of the Association.

Pauline Byakika–Kibwika (née Pauline Byakika), is a Ugandan specialist physician, internist, epidemiologist, academic and researcher, who serves as an Associate Professor of Medicine at Makerere University College of Health Sciences. From 2017 until 2019, she served as the Vice President of the Uganda Medical Association, a professional industry association, that champions medical doctors' interests in the county.

She was born in the Eastern Region of Uganda circa 1973. After attending local schools, she was admitted to the Makerere University, where she studied human medicine. She graduated with a Bachelor of Medicine and Bachelor of Surgery (MBChB) degree in 1999. She followed that with a Master of Medicine (MMed) degree in Internal Medicine, also from Makerere University. Her third degree was a Master of Science (MSc) in Clinical Epidemiology and Biostatistics, obtained from Makerere University. She also holds a Doctor of Philosophy (PhD) in Clinical Pharmacology obtained from the Trinity College Dublin, in Dublin, Ireland. Her PhD thesis analyses the drug-to-drug interactions between antimalarials and Anti-retroviral drugs.